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Nomenclature of pCAMBIA GusPlus vectors

The numbering system works as follows:

  • First digit - indicates plant selection: 0 for absence; 1 for hygromycin resistance; 2 for kanamycin.
  • Second digit - indicates bacterial selection: 1 for spectinomycin/streptomycin resistance; 2 for chloramphenicol; 3 for kanamycin; 4 for spec/strep and kanamycin.
  • Third digit - indicates polylinker used: 0 for pUC18 polylinker; 8 for pUC8 polylinker; 9 for pUC9 polylinker.
  • Fourth digit - indicates reporter gene(s) present: 0 for no reporter gene; 1 for E.coli gusA; 2 for mgfp5; 3 for gusA:mgfp5 fusion; 4 for mgfp5:gusA fusion; 5 for Staphylococcus sp. gusA (GUSPlus).
  • Fifth digit - the .1 denotes the absence of a signal peptide from the GUSPlus protein and the .2 denotes the presence of the GRP signal peptide for in planta secretion of the GUSPlus protein.

Quick-pick table (clicking on a plasmid number will navigate to the Genbank file)

pCAMBIA

Plant Selection Gene

Bacterial Selection Gene

Polylinker

Reporter Gene

T-DNA size (bp)

Reading Frame or lacZ

Vector family

Map

1305.2

hptII

kan

pUC18

gus plus with secretion signal peptide

5667

-

GIS

view map

0305.2
(identical to above vector but contains no hptII cassette)

-

kan

pUC18

gus plus with secretion signal peptide

3412

lacZ

GIS

1305.1

hptII

kan

pUC18

gus plus

5592

-

GIS

view map

0305.1
(identical to above vector but contains no hptII cassette)

-

kan

pUC18

gus plus

3408

lacZ

GIS

view map

1105.1 (download as attachment)

hptII

spec/strep

pUC18

gus plus

5854

lacZ

GIS

view map

1105.1RC

hptII

spec/strep

MCS with rare cutting enzymes

gus plus

3654

GIS


(info)

1105.1U

hptII

spec/strep

unique MCS

gus plus

5830

view map (info)

1105.1R
(download as attachment)

hptII

spec/strep

modified MCS

gus plus

5915

-

GIS

view map

1105.1RRC

hptII

spec/strep

MCS with rare cutting enzymes

gus plus

3665

GIS


(info)

0105.1R
(identical to above vector but contains no AseI-hptII cassette, but if you have VectorNTi program you can download the attached sequence as gb.file)

-

spec/strep

gus plus

3489

-

GIS

view map