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Nomenclature of pCAMBIA GusPlus vectors
The numbering system works as follows:
- First digit - indicates plant selection: 0 for absence; 1 for hygromycin resistance; 2 for kanamycin.
- Second digit - indicates bacterial selection: 1 for spectinomycin/streptomycin resistance; 2 for chloramphenicol; 3 for kanamycin; 4 for spec/strep and kanamycin.
- Third digit - indicates polylinker used: 0 for pUC18 polylinker; 8 for pUC8 polylinker; 9 for pUC9 polylinker.
- Fourth digit - indicates reporter gene(s) present: 0 for no reporter gene; 1 for E.coli gusA; 2 for mgfp5; 3 for gusA:mgfp5 fusion; 4 for mgfp5:gusA fusion; 5 for Staphylococcus sp. gusA (GUSPlus).
- Fifth digit - the .1 denotes the absence of a signal peptide from the GUSPlus protein and the .2 denotes the presence of the GRP signal peptide for in planta secretion of the GUSPlus protein.
Quick-pick table (clicking on a plasmid number will navigate to the Genbank file)
|
pCAMBIA |
Plant Selection Gene |
Bacterial Selection Gene |
Polylinker |
Reporter Gene |
T-DNA size (bp) |
Reading Frame or lacZ |
Vector family |
Map |
|---|---|---|---|---|---|---|---|---|
|
hptII |
kan |
pUC18 |
gus plus with secretion signal peptide |
5667 |
- |
GIS |
||
|
0305.2 |
- |
kan |
pUC18 |
gus plus with secretion signal peptide |
3412 |
lacZ |
GIS |
|
|
hptII |
kan |
pUC18 |
gus plus |
5592 |
- |
GIS |
||
|
0305.1 |
- |
kan |
pUC18 |
gus plus |
3408 |
lacZ |
GIS |
|
|
1105.1 (download as attachment) |
hptII |
spec/strep |
pUC18 |
gus plus |
5854 |
lacZ |
GIS |
|
|
1105.1RC |
hptII |
spec/strep |
MCS with rare cutting enzymes |
gus plus |
3654 |
GIS |
(info) |
|
|
1105.1U |
hptII |
spec/strep |
unique MCS |
gus plus |
5830 |
|||
|
1105.1R
|
hptII |
spec/strep |
modified MCS |
gus plus |
5915 |
- |
GIS |
|
|
1105.1RRC |
hptII |
spec/strep |
MCS with rare cutting enzymes |
gus plus |
3665 |
GIS |
(info) |
|
|
0105.1R
|
- |
spec/strep |
gus plus |
3489 |
- |
GIS |
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