Nomenclature of Legacy pCAMBIA vectors

The four digit numbering system works as follows:

• First digit - indicates plant selection: 0 for absence; 1 for hygromycin resistance; 2 for kanamycin; and 3 for phosphinothricin (the vectors containing the phosphinothricin resistance gene are no longer available from CAMBIA at the request of Bayer, which owns patents restricting its use in some countries).
• Second digit - indicates bacterial selection: 1 for spectinomycin/streptomycin resistance; 2 for chloramphenicol; 3 for kanamycin; 4 for spec/strep and kanamycin.
• Third digit - indicates polylinker used: 0 for pUC18 polylinker; 8 for pUC8 polylinker; 9 for pUC9 polylinker.
• Fourth digit - indicates reporter gene(s) present: 0 for no reporter gene; 1 for E.coli gusA; 2 for mgfp5; 3 for gusA:mgfp5 fusion; 4 for mgfp5:gusA fusion; 5 for Staphylococcus sp. gusA (GUSPlus).
• Fifth digit - notes some other special feature. So far this has been used only with: pCAMBIA1305.1 and plasmids derived from it, where the .1 denotes the absence of a signal peptide from the GUSPlus™ protein; and pCAMBIA1305.2 where the .2 denotes the presence of the GRP signal peptide for in planta secretion of the GUSPlus™ protein.
• Lagging letter - X indicates that the reporter gene lacks its own start codon and the vector is for creating fusions to the reporter; Z indicates presence of a functional lacZa for blue-white screening; a/b/c indicates the reading frame for fusions with the Fuse and Use vectors.

Notes

• Due to resource limitations, not all possible vector feature combinations have been created at CAMBIA. You may initially be disappointed to find that we don't have, for example, a pCAMBIA2205.2. The vectors were designed however, such that it should be a relatively simple matter for a researcher needing such a vector to construct it from the components in other vectors.
• If you have created a pCAMBIA vector derivative that other researchers will find useful, please let us know .
• Vector Manual Addendum: Important note to pCAMBIA users

Quick-pick table

(clicking on a plasmid number will navigate to the Genbank file)

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