How does DArT work?

Note: This technology is available for use to anyone that agrees to the improvement-sharing conditions of a royalty-free BiOS Genetic Resources Indexing Technology License.

The process involves the development of a genotyping array.

Genotypes for each DArT marker are then scored using hybridisation with the genotyping array. This can be done in a variety of different ways. In the most common implementation, the genomic representation fragments are stained with a dye that fluoresces green, while vector sequences are stained with a dye that fluoresces red, so that image analysis after the hybridisation, wash and scan can be done by comparison of the intensity of green and red fluorescence as in a typical expression microarray. The outcome is the genotype of the sample: a list of array features (spots) hybridising to the sample. The genotype is a table showing presence (1) or absence (0) for each marker. Any standard genetic analysis software can then be used to analyse the data.

The first example of Diversity Arrays was published by Jaccoud et al., Nucleic Acids Research in February 2001, applied to rice, and the genome complexity reduction technique was derived from AFLP®. For most applications, this would not provide sufficiently complex representations, so we are interested in improvements. One of the major purposes of an open source license is to provide for grantback of improvements to all licensees, not just to the owner or the improver of the technology. Although CAMBIA does not require BiOS licensees to pay royalties, all licenses to DArT contain open source provisions that are required for compliance to maintain the license.

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